Our team have successfully inserted the tetO2-GFP sites (tetO2-0 EGFP , tetO2-0 sfGFP and tetO2-1 EGFP) into the E.Coli MG1655 genome (attTn7 site) by recombination with our designed plasmid containing the tet-O2-GFP site.
Subsequently, we transformed our silencer plasmid into these tetO2-GP strains and the GFP intensity is measured.
The GFP intensity results are as follow:
The untransformed E.Coli MG1655 (tetO2-0 EGFP, tetO2-0 sfGFP or tetO2-1 EGFP incorporated into the genome) is set as control and the fluorescence intensity is calibrated as 100%.
Note that the data collected are considered only when difference between values have the P-value is less than 0.05.
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