- Data For Our Favorite New Parts:
1. Main Page –oriR101 & repA101-ts, BBa_K524000: This heat sensitive origin of replication origin has been shown to be function at 30°C Partial loss of plasmid happens at 33°C, and complete loss of plasmid is observed at 37°C,
- Parts Constructed But Not Characterized
1. Main Page –pLac + RBS + sfGFP1-10, BBa_K524001: This contains coding DNA sequences for superfolder GFP amino residues 1-214 driven by RBS BBa_B0034 and pLac BBa_R0010.
2. Main Page –pLac + RBS + sfGFP11 + double terminator, BBa_K524002: This contains coding DNA sequences for superfolder GFP amino residues 215-238 driven by RBS BBa_B0034 and pLac BBa_R0010, with double terminator B0015 at the end.
3. Main Page – nadE gene + double terminator, BBa_K524003: an essential gene that encodes constitutive expressed NAD+ synthatase.
4. Main Page –pir gene, BBa_K524004: the pir gene encodes the autogenously regulated pi protein.
5. Main Page – pToolkit, BBa_K524005: A plasmid that is only maintained in strains with genotype pir+. The plasmid was modified from another plasmid pKD46, which has been shown to trigger DNA recombination and subsequent gene knockout under induction of arabinose.
6. Main Page –RBS + sfGFP1-10, BBa_K524006: This contains coding DNA sequences for superfolder GFP amino residues 1-214 driven by RBS BBa_B0034.
7. Main Page –RBS + sfGFP11 + double terminator, BBa_K524007: This contains coding DNA sequences for superfolder GFP amino residues 215-238 driven by RBS BBa_B0034, with double terminator B0015 at the end.
8. Main Page – bcr CDS + double terminator B0015, BBa_K524100: The gene product is responsible for multidrug efflux system that pumps out antibiotics. Effect of pump was documented by other researchers: it confers varying degree of resistance to 5 antibiotics.
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