1. Background
2. Building the horse - Engineering a novel strain for antibiotics-free selection
3. The army inside - Toluene-4-Monooxygenase (T4MO)
Toluene-4-Monooxygenase (T4MO) is a three-component enzyme system that is naturally able to degrade a variety of hydrocarbons. In particular, its ability to oxidize and reduce small aromatic compounds has caught the attention of scientists looking for biological means of producing certain useful organic chemicals for industrial use, resulting in extensive research on modifying its activity and affinity.
For our project, we have chosen a mutant form of T4MO,
3.1 Soldiers need shields...maybe - Multidrug Efflux Pump (Bcr)
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