jun 28-july 2

2011.6.28

---

cultivate the bacteria of cheZ deficiency(RP1616),and the Control group(RP437). check the resistibility of RP1616 and RP437 result:both of the two groups are of none resistibility. members:Siwei Luo, Fangzhou Zhao, Zhilin Chen...

2011.6.29

---

check the motility of RP1616 strain and RP437 strain cultivate Top10 strain result: the bacterial plaque of RP1616 is smaller than that of RP437, implying that RP1616 is of cheZ deficiency, causing the decreased motility of the bacteria.

6.30

---

prepare for the competent cell of Top10 strain cultivated on 6.29 extract the genome of Top10 strain and use it as complates to run PCR of CheZ result: the concentration of PCR result is too low to continue the experiments.

7.1

---

conduct PCR of CheZ again ligate the CheZ DNA segment to plasmid PSb1C3, and transform it into Top10 competent cells result:as the picture shows.

7.2

---

pick up single colony from the Petri dish to reproduce, in which the competent cells are cultivated and use them colony PCR result:The bacteria grow up well on dishes with chloromycetin resistency，verifying that the transformation is successfull while the concentration of PCR product is pretty high,demonstrating that the plasmids indeed contains the sagment CheZ.

7.3

---

extract the plasmids from the reproduced bacteria and send it to check the base sequence result: the concentration of the plasmids extracted is about 500ng/uL

7.4

---

extract the standard parts LuxPR and Terminator from plate sent by iGEM registry 2010 and put them into Top10 strain by transformation (LuxPr: Plate 2,24C Terminator : Plate 1,6O) result: the bacteria on the plate with Amp resistency grows well

July 3-July 9

7.5

---

pick up single colony from the Petri dish to reproduce in the liquid culture medium

7.6

---

extract plasmids containing LuxPR and Terminator and make enzyme digestion with EcoR1 and Spe1 in order to validate the existence of the two parts recieve part Toggle Switch and rbs-Ci-ter from PKU and conduct transformation

result: Due to the short length of LuxPR and Terminator, the result of the enzyme digestion is hard to examine by electrophoresis.

7.7

---

result: there are a few colonies with part Toggle switch grown while no colony of rbs-Ci-ter appeared.

7.8

---

pick up single colony with part Toggle switch . extract the standard parts LuxPR and Terminator from plate sent by iGEM registry 2011 and put them into Top10 strain by transformation

7.9

---

pick up single colony with LuxPR & terminator. extract plasmids containing toggle switch to conduct PCR.

process PCR with plasmids aptamer-CheZ result:as the picture shows. the base sequence of CheZ extracted before is proved right.

7.10

---

ligate aptamer-CheZ with PSB1C3 plasmids

extract plasmids containing LuxPR and terminator result:the concentractions of plasmids LuxPR and terminator are both 600ng/ul

7.11

---

transform PSB1C3 containing aptamer-CheZ into bacteria and cultivate.

July 10-July 16

7.12

---

carry out Double digestion of toggle switch and pSB1c3 and ligate them together.

pick up single colony from bacteria cultivated yesterday.

result: Both the location and the brightness of the electrophoretic bands correspond with our expectation.

7.14

---

transform the part rbs-ci-ter sent from PKU again into bacteria. check the colors of the colonies containing toggle switch. result: the ratio of red vs green is 8:25, verifying the function of toggle switch.

7.15

---

pick up the single colony from the plate yesterday and ten hours later extract the plasmids containing Ci-terminator.

7.16

---

conduct PCR with the plasmids yesterday to reproduce rbs-ci-Term. result:failure

7.18-7.21

---

ligate the standard part Terminator to the end of toggle switch result: we conduct a enzyme digestion with EcoR1 and Pst1 to the final ligation product and the electrophoresis result shows that the ligation is successful.

July 17-July 23

July 24-July 30

July 31-Aug 6

Aug 7-Aug 13

Aug 14-Aug 20

Ning Wang

Yuntao Liu

Dan Zhou

Name:Dan Chou
Email: zhoudan@mail.ustc.edu.cn
Study field: Chemistry
Interests: Music&Movies
Research Interest: Biochemistry
I am the clerk and the manager of USTC-China 2011 iGEM team, and I am the secretary-general of 2011 iGEM China-meetup. I also participated in the experiment of constructing the prompter, aptamer, cheZ and GFP together. I feel this enjoyable all the time.

Linna An

Hi,I'm Anlina,and my Chinese name is also Linna An.I am now a new sophomore in USTC,the department of Chemistry and Material Science.I designed the wiki,completed it with my friend Yuntao Liu,and did some experiments with other teammates.But the most valuable experience is that I participated a complete project,which will definately help with my career.Not everything is easy,but there is always others to help.Not every day is happy,yet all the difficulties made what we are.When there is will,there is way!

Junhui Peng

Junhui Peng Senior undergraduate in Lab of Computational Biology, School of Life Sciences, USTC. I think, it must be very exciting to find and understand the principles of how macromolecules organize in universe. In iGEM lab and compubio lab, you can try to build your own principles for bacteria and for macromolecules. Badminton is my favorite sport, and Defense of the Ancients is my favorite computer game.

Yanyue Wang

I am Wang Yanyue from Department of Material Science and Engineering. You can call me Era for short. In our team I am in charge of the Human Practice part and also make my contribution to performing some experiments. The reason why I joined the team was that biology is so mysterious and fancy for an outsider like me, and I wish one day I may be able to untangle some of the fascinating enigmas of living creatures.Well, it is a bliss that we are living in such a wonderful world with so many lovely people caring about each other. We are so young and have the hearts to live what seemed impossible and challenging. As long as we have enough courage and wit, we are the ones who can make a change!

Xiaolei Zhang

My name is Xiaolei Zhang,an undergraduate of system biology in USTC. While I began to cast doubt on the my conviction of studying biology,synthetic biology with the iGEM came to touch me, fascinating me with its innovative application of engineering idea in biology.In retrospect,all the diverse elements in this iGEM trip make me to see biology and scientific research from a refreshing point of view.Anyway,whether our research is serious enough,I take it seriously and cherish this iGEM trip.

Yang Zhou

Yanyue Wang

I am Wang Yanyue from Department of Material Science and Engineering. You can call me Era for short. In our team I am in charge of the Human Practice part and also make my contribution to performing some experiments. The reason why I joined the team was that biology is so mysterious and fancy for an outsider like me, and I wish one day I may be able to untangle some of the fascinating enigmas of living creatures. Well, it is a bliss that we are living in such a wonderful world with so many lovely people caring about each other. We are so young and have the hearts to live what seemed impossible and challenging. As long as we have enough courage and wit, we are the ones who can make a change!