Team:ZJU-China/Notebook/September

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This group...........

In this month we......
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Day

Note

Aug.29th Monday

• Meeting. Summarize all the experiment work so far.

• Cut: 22M(X+P), 1C3(E+P)

Aug.30th Tuesday

Aug.31st Wednesday

• Cut vgb+YFP+tetR, fdhF+RFP+tetR.
• Run the digestion results

• Culture the positive results

Sept.1st Thursday

• Miniprep: Y#4, R#2/3/4/5
• Cut Y#4, R#2/3/4/5

•Run the gel.
• Send R#3,Y#4 sample to sequencing

• Hypoxia culture: RFP3, RFP4
• Preserve Y#4, R#2/3/4/5

Sept.2nd Friday

• Phusion PCR

• Hypoxia Culture: R, Y
• Run the PCR results. Bands are confirmed right.

• Purification: vgb, YFP, tetR.

Sept.3rd Saturday

• Culture: YFP, RFP, A25
• Culture in micro-oxygen: YFP, RFP, A25

• Culture in slope medium: RFP, YFP, A25
• Hypoxia culture: RFP, YFP, A25

• Check the fluorescence.
• Neither RFP in hypoxia or oxygen condition. Little YFP both in hypoxia and oxygen.

Sept.4th Sunday

• Miniprep: RFP3, YFP4

• Cut: RFP3, YFP4, !C3.