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From 2011.igem.org

いんぐりっしゅ！

Ligation

Refer to following table, prepare a reaction solution.

insert	0.5 µl
vector	0.5 µl
2 x Buffer	2.5 µl
T4 ligase	0.5 µl
H2O	1.0 µl
	total 5 µl

Incubate it for 30min at 16 ℃.