Team:NCTU Formosa/RNA discussion

From 2011.igem.org

Revision as of 09:50, 2 October 2011 by Minhua (Talk | contribs)



RNA Thermometer

Discussion

Our experimental results indicate that high temperature decreased the translation rate of the target protein, and this temperature-dependent genetic circuit can control the expression level of the target protein by the host cell's incubation temperature. However, the translational activity of the BBa_K115002 at different temperatures cannot be quantified directly from experimental data. To overcome this problem, we provided a dynamic model which can quantitatively assess the translation strength of the BBa_K115002 at temperatures 27°C, 32°C, 37°C and 42°C.