Team:WHU-China/Parts/Data

Data for our favorite part

BBa_K560000 Main page - Blue Light Sensing and Reporting System: This part shows the complete system of light sensing and reporting regulation in E.coli. Although it is not very stable, it can truly work in TrpR(-) E.coli.

Data for pre-existing parts

1. Experience – BBa_I15010 Cph8 (Cph1/EnvZ fusion) (UTAustin, iGEM2004): This part was used by 2011 WHU_China team as a part of light sensing system. However, the result of sequencing proves that this part in the 2011 kit turns out to be a vector without the fragment of cph8. 2011 WHU_China requested the same part from the 2011 kit of HUST_China, but the result of pcr and restriction enzymes cleavage shows that their plasmids have the same problem.
2. Experience – BBa_E0040 Green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (Endy Lab, iGEM2004): This part was used by WHU_China team as a reporter of long-term oscillator. Information from the website of this part shows that its length is about 720bp; however, the result of restriction enzymes cleavage experiment showed that it is actually about 1kb. And after several attempts, we failed to assemble this part with other biobricks.

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