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From 2011.igem.org

Monday

Today was a very dissapointing day. We have not been able to grow any cultures with our CYPs. But we'll try again!

And we are not completely useless cause we have managed to produce competent cells and amplify an ekspression vector from the iGEM kit. Hurra.

Lab Work

• Did point mutations - see protocol here: Mutations

• Used our mutated plasmids from last week to transform competent XL1-Blue cells. Again. But this time we used 10μL of our plasmids instead of 1μL. See protocol here: Transformations

Other

• Worked on our wiki
• Enjoyed the good weather