Building a Naphthenic Acid Biosensor

The Design

Figure 1 In order to develop a naphthenic acid biosensor we were required to develop a sensory element which would respond specifically to this class of compounds. Additionally, we developed a novel electrochemical reporter system using the lacZ gene product Beta-galactosidase to detect and quantify the response of our promoter into a measurable system. Since E. coli is not naturally found in tailings ponds, we designed a novel conjugation system to transfer our reporter system into Pseudomonas as well as protocols for use of our system in microalgae.

Figure 2 Our vision was to use our construct in a device which could be used to quantitate the amount of naphthenic acids in a tailings pond solution. A sample would be loaded into the device, the bacteria would respond to the naphthenic acids in solution and produce an electrochemical output. This could be quantitatively measured and the output graphed onto a computer.

Data From New Parts Submitted To The Registry

Bba_K640002, Bba_K640003, Bba_K640004, Bba_K640005 - These parts contain oriT, ori1600, oriT-ori1600, and oriT(Heidelberg Part)-ori1600 respectively. We have characterized the expression of these parts on our Pseudomonaschassis section of the wiki.
Bba_K640000, and Bba_K640001 - Codon optimized Luciferase and Hsp70A/RbcS2 Microalgae parts. More information can be found in our microalgae chassis section.

Data From Characterized Parts Already In the Registry

Additional Work and Characterization

Optimized K300022 Part For Transformation Into Microalgae (See Experience Page)
Characterized the Breakdown of CPRG to CPR using spectrometry over time BBa_I732901 (See Experience Page)

Team:Calgary/Project/DataPage

From 2011.igem.org

The Project

Building a Naphthenic Acid Biosensor

The Design

Data From New Parts Submitted To The Registry

Data From Characterized Parts Already In the Registry

Additional Work and Characterization