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From 2011.igem.org

Parts submitted

Part Registry

Name	Description	Type	Designer	Length	Lenght (prefix;suffix)
BBa_K587000	RecA	Protein coding	David Maycotte/Patricia Campos	1062	1103
BBa_K587001	Crxst	Regulatory	David Maycotte	35	78
BBa_K587002	Tast	Regulatory	David Maycotte	80	123
BBa_K587003	Tawk	Regulatory	David Maycotte	79	122
BBa_K587004	Crxwk	Regulatory	David Maycotte	35	78
BBa_K587005	Itast	Regulatory	David Maycotte	81	124

Sequences and description

• [http://partsregistry.org/Part:BBa_K587001 RecA]

Protein coding sequence

Sequence

GAATTCGCGGCCGCTTCTAGATGGCTATCGACGAAAACAAACAGAAAGCGTTGGCGGCAGCACTGGGCCAGATT GAGAAACAATTTGGTAAAGGCTCCATCATGCGCCTGGGTGAAGACCGTTCCATGGATGTGGAAACCATCTCTACCGGTTCGCTTTCACTGGATAT CGCGCTTGGGGCAGGTGGTCTGCCGATGGGCCGTATCGTCGAAATCTACGGACCAGAATCTTCCGGTAAAACCACGCTGACGTTGCAGGTGATCGCCGC AGCGCAGCGCGAAGGTAAAACCTGTGCGTTTATCGATGCTGAACACGCGCTGGACCCAATCTACGCACGTAAACTGGGCGTCGATATTGACAACCTGCT GTGCTCCCAGCCGGATACCGGCGAGCAGGCACTGGAAATCTGTGATGCCCTGGCACGTTCTGGCGCAGTAGACGTTATCGTCGTTGACTCCGTGGCGGCAC TGACGCCGAAAGCGGAAATCGAAGGCGAAATCGGCGACTCTCACATGGGCCTTGCGGCACGTATGATGAGCCAGGCGATGCGTAAGCTGGCGGGTAACCTG AAGCAGTCCAACACGCTGCTGATCTTCATCAACCAGATCCGTATGAAAATTGGTGTGATGTTCGGTAACCCGGAAACCACTACCGGTGGTAACGCGCTGAAA TTCTACGCCTCTGTTCGTCTCGACATCCGTCGTATCGGCGCGGTGAAAGAGGGCGAAAACGTGGTGGGTAGCGAAACCCGCGTGAAAGTGGTGAAGAACAAA ATCGCTGCGCCGTTTAAACAGGCTGAGTTCCAGATCCTCTACGGCGAAGGTATCAACTTCTACGGCGAGCTGGTTGACCTGGGCGTGAAAGAGAAGCTTATC GAGAAAGCAGGCGCGTGGTACAGCTACAAAGGTGAGAAGATCGGTCAGGGTAAAGCGAATGCGACTGCCTGGCTGAAAGATAACCCGGAAACCGCGAAAGAG ATCGAGAAGAAAGTACGTGAGTTGCTGCTGAGCAACCCGAACTCAACGCCGGATTTCTCTGTAGATGACAGCGAAGGCGTAGCAGAAACTAACGAAGATTT TTAATACTAGTAGCGGCCGCTGCAG

• [http://partsregistry.org/Part:BBa_K587002 Crxst]

This DNA sequence acts as a lock for the low arabinose concentration regulation path.

Sequence

GAATTCGCGGCCGCTTCTAGAGAAGCGCAGTAATTCACCTCTTGGATTTGGGTATCATACTAGTAGCGGCCGCTGCAG

• [http://partsregistry.org/Part:BBa_K587003 Tast]

This sequence acts as the key for the Crxst lock and it unlocks it, in order to activate following mechanism.

Sequence

GAATTCGCGGCCGCTTCTAGAGAGATCTCTATATACCATACCACCAATTAAAAAGTAATTGGTGGTTTCGCGTCATTAAGTGGAGAACCTAAAGGGATAGTT TACTAGTAGCGGCCGCTGCAG

• [http://partsregistry.org/Part:BBa_K587004 Tawk]

This sequence acts as the key fot Crxwk lock and it unlocks it, in order to activate the following mechanism.

Sequence

GAATTCGCGGCCGCTTCTAGAGAGATCTCTATATACCATACCACCAATTAAAAAGTAATTGGTGGTTTCGCGTCATTAAGTGGAGATAAACCGTAAGGGTT TACTAGTAGCGGCCGCTGCAG

• [http://partsregistry.org/Part:BBa_K587005 Crxwk]

This DNA sequence acts as a lock for the high arabinose concentration regulation path.

Sequence

GAATTCGCGGCCGCTTCTAGAGAAGCGCAGTAATTCACCTCTATTTGGCATTCCCAATACTAGTAGCGGCCGCTGCAG

• [http://partsregistry.org/Part:BBa_K587006 Itast]

This is a complementary sequence for the Tast key, so we call it the antikey since it will inactivate the Tast key and preventing it to interact with the Crxst lock.

Sequence

GAATTCGCGGCCGCTTCTAGAGTCTAGGAGAGATATATGGTATGGTGGTTAATTTTCATTAACCACCAAAGCGCAGTAATTCACCTCTTGGTTTCCCTATCAA TACTAGTAGCGGCCGCTGCAG