Team:Caltech/Recipes
From 2011.igem.org
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50% glycerol Stock:
Agar/LB plate (Autoclaved):
Ampicillin Stock
Chloramphenicol Stock
Enrichment Minimal Media
Gibson Mix (1.33x)For 25 aliquots of 15 μl each:
IPTG stockFor 1000x stock (.1M)
Ligation ReactionFor a 20 uL reaction:
p450 degradation testing solutionFor a 200uL reaction:
Phusion PCRFor a 50uL reaction:
SOC Mediafor 250 ml (adapted from [http://openwetware.org/wiki/SOC OpenWetWare]):
Restriction DigestFor a 50uL reaction:
Taq PCR (for 16s insert)For a 25uL reaction:
X-gal stock (50x)For 20mg/mL, total 0.5mL volume:
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