Team:UC Davis/Notebook wip

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Criteria

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Contents

Week 1

-Rehydrated parts from 2011 Registry Distribution:

    • -R0040=Tet promoter in psb1a2
    • -C0040=Tet repressor in psb1a2
    • -C0012=LacI repressor in psb1a2
    • -C0051=Lambda cI repressor in psb1a2
    • -R0051=cI-regulated promoter in psb1a2
    • -I732006=LacZ-alpha in psb1ak3
    • -R0010=LacI regulated promoter in psb1a2
    • -E0040=GFP coding region in psb1a2
    • -J23101=Constitutive promoter in j61002
    • -C0080=AraC repressor/activator in psb2k3
    • -I13458=pC+AraC in psb1a3
    • -I13453=pBAD promoter in psb1a3
    • -B0015=Double terminator in psb1ak3

-Transformed all hydrated parts -Cultured all parts and digested to check on a gel. All parts checked out. Sent out all hydrated parts to get sequenced. -Ligated mutants into screening plasmid to see if our error-prone PCRs had visible mutants. -Ordered primers for mutagenesis of LacI promoter, cI promoter and tet promoter. Also ordered primers to replace RBS (Bba_B0032) in Bba_E0240 to Bba_B0034.

Week 2

Week 3

Week 4

Week 5

Week 6

Week 7

Week 8

Week 9

Week 10

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