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Team:Arizona State/Templates/E coli contingency/table 4
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Revision as of 13:33, 28 September 2011 by Ethan ward (Talk | contribs)
| E. Coli Strain | Initial Plasmid | Introduced Plasmid | Antibiotic selection | DNA Quantity Used (ng) | # of Colonies | Weighted Colony Count** |
|---|---|---|---|---|---|---|
| MG1655 | GFP Construct in pRSF | Leader in pIDT | Amp | 140 | 21 | 138.742 |
| MG1655 | GFP Construct in pRSF | Leader+Seq1 in pIDT | Amp | 147 | 31 | 204.810 |
| MG1655 | Leader in pIDT | GFP Construct in pRSF | Kan | 143.4 | 23 | 117.635 |
| MG1655 | Leader+Seq1 in pIDT | GFP Construct in pRSF | Kan | 143.4 | 1000 | 3909.508 |
| BL21 | GFP Construct in pRSF | Leader in pIDT | Amp | 140 | 0 | 0.000 |
| BL21 | GFP Construct in pRSF | Leader+Seq1 in pIDT | Amp | 147 | 2 | 11.574 |
| BL21 | Leader in pIDT | GFP Construct in pRSF | Kan | 143.4 | 14 | 60.512 |
| BL21 | Leader+Seq1 in pIDT | GFP Construct in pRSF | Kan | 143.4 | 9 | 35.186 |
**Weighted Colony Count = (# of Colonies)/(mean OD600 of competency prep)
