Wildtype

SEQUENCE GOES HERE! 0, .0014, .0028, .0042, .0056, .0070, .0140 0.99566, 0.95671, 0.880328, 0.734557, 0.662629, 0.607491, 0.530352 0.012905, 0.015917, 0.020404, 0.026943, 0.013849, 0.0068952, 0.0060456 4

Mutant 1

SEQUENCE GOES HERE! 0, .0014, .0028, .0042, .0056, .0070, .0140 0.6492, 0.20526, 0.17329, 0.16899, 0.16682, 0.16631, 0.16913 0.017682, 0.0032754, 0.0010138, 0.0011266, 0.00097033, 0.0012072, 0.0011179

Mutant 2

SEQUENCE GOES HERE! 0, .0014, .0028, .0042, .0056, .0070, .0140 0.53303, 0.16064, 0.14171, 0.12149, 0.12547, 0.11146, 0.1061 0.02651, 0.0029121, 0.0027198, 0.0033112, 0.0034421, 0.0059837, 0.0027095

Mutant 4

SEQUENCE GOES HERE! 0, .0014, .0028, .0042, .0056, .0070, .0140 0.23, 0.038825, 0.035663, 0.036119, 0.035423, 0.034289, 0.032858 0.02048, 0.00047265, 0.00028731, 0.00037973, 0.00031652, 0.00026622, 0.00070058

Mutant 5

SEQUENCE GOES HERE! 0, .0014, .0028, .0042, .0056, .0070, .0140 0.92311, 0.23346, 0.1833, 0.17092, 0.16534, 0.16473, 0.15538 0.0061434, 0.0029621, 0.0016015, 0.0037633, 0.0029015, 0.002096, 0.0024785

Mutant 6

SEQUENCE GOES HERE! 0, .0014, .0028, .0042, .0056, .0070, .0140 0.97182, 0.98592, 1.01000, 1.02300, 1.03610, 1.04490, 1.01750 0.018621, 0.01966, 0.018634, 0.018826, 0.018993, 0.020762, 0.019842

Mutant 7

SEQUENCE GOES HERE! 0, .0014, .0028, .0042, .0056, .0070, .0140 0.48325, 0.13919, 0.12353, 0.10617, 0.10943, 0.099213, 0.08565 0.012073, 0.0025601, 0.0030506, 0.0027667, 0.002179, 0.0022998, 0.0015159

Mutant 8

SEQUENCE GOES HERE! 0, .0014, .0028, .0042, .0056, .0070, .0140 0.41657, 0.12325, 0.10744, 0.10365, 0.10006, 0.096006, 0.09219 0.0080566, 0.0028055, 0.0027614, 0.0038199, 0.0038188, 0.0024541, 0.0022523

The above widget displays the relative GFP fluorescence data that we have collected for the 9 lambda cI mutant promoters and the wild type R0051 promoter. The plots are normalized to the wild type such that the wild type's fluorescence at 0% arabinose and 0 mM IPTG is 1. The data for these plots were gathered using the constructs outlined on the lambda cI project page, which were designed so that increasing the arabinose concentration would proportionally increase the repressor concentration.