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Team:MIT/Tools/
From 2011.igem.org
''mcell'' - A Multicellular Modeling Framework
''mcell'' is a small set of Python classes that allows the enterprising modeler to: * Easily create very flexible models of of multicellular dynamics * Manage the models already created through a simple command-line interface * Easily change defined parameters in models * Render the runs of the models in a convenient, simple way * Analyze the runs of the models through histograms and clustering ''mcell'' is a thin layer on top of the combination of [BionetSolver], which model internal cell states as systems of ODEs, and [CompuCell3D], which models cell shape dynamics using the Glazier-Graner-Hogeweg methos. It was created largely in reaction to inconveniences experienced when using BionetSolver and CompuCell3D as detailed [here]. It is in continuous development.Quick Links
Why?
Over the course of the summer, we tried several ways of modeling multicellular dynamics. The first thing we tried was everyone's trusted favorite, MATLAB. We generated a system of several thousand ODEs, in which groups of 5 ODEs represented the state of a single cell, and were coupled to ODEs describing adjacent cells. Unfortunately, this was very, very slow, and did not allow for the modeling of cell shape dynamics.
Then, we discovered a way to couple CompuCell3D with ODE models of internal cell states. We realized that, if we wanted to do any sort of reasonably flexible modeling, we needed to do something like this. So, we downloaded CompuCell3D, read the documentation, and started modeling various circuits.
Now, CompuCell3D coupled with BionetSolver is a great system. However, we quickly discovered that this wasn't quite enough for our purposes. For instance, CompuCell3D allows one to dynamically change the "cell type", and this the cell color. However, as we were trying to engineer cells, we were interested in the dynamics of the protein expression levels -- in fundamentally continuous quantities -- rather than a discrete notion of "cell type"! CompuCell 3D would not let us visualize the values that we needed -- so we wrote a Python script to render the models the way we wanted to.
Other problems emerged. We wanted to be able to analyze the data in other ways -- in particular, we wanted to see histograms of the levels of expression of some protein. We wanted to be able to cluster cells by the dynamics of some protein. Both of these problems led to more Python scripts. In addition, we realized that
Installation
How it works
In mcell models, BionetSolver models the internal cell states, and CompuCell3D models the cell dynamics.BionetSolver
BionetSolver reads one or several [SBML] files, each of which define a ''circuit'' as a system of chemical reactions in several containers with given rate laws. SBML models are simply XML files, and can be written with a text editor; however, it is much easier to define them using a graphical designer, like [JDesigner], or using a simple scripting language, like [Jarnac]. (Both of those editors can be obtained by installing the [Synthetic Biology Workbench]. After loading the circuits, BionetSolver is in posession of a system of ODEs that define the internal state of each modeled cell. To simulate each cell, BionetSolver simply Euler-steps its ODE system forward with a fixed time step. Some of the variables in the ODE model (like, say, the concentration of a protein on the neighboring cells) are actually parameters that are continually updated from the CompuCell3D thread.CompuCell3D
Model Management
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Rendering and Analysis
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