Team:Colombia/Notebook/Protocols

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PCR for 16s (10 µl)

Reactives	µl	µl
Buffer	2.5	1
MgCl2	2	0.8
DNTP´s	0.5	0.2
P1492R	0.5	0.2
P27F	0.5	0.2
Taq	0.25	0.1
DNA	2	0.8
H2O	17.75	6.8
Final Vol	26	10

PCR Pfx

|Reactives |ul |- |H2O |17.2 |- |Buffer PFX |3 |- |MgSO4 |0.6 |- |dNTP´s |0.9 |- |FW |0.9 |- |RV |0.9 |- |Enhancer |3 |- |Taq PFX |0.5 |- |ADN |3 |- |Total Mix |30