Team:Colombia/Notebook/Protocols

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PCR for 16s (10 µl)

Reactives	µl	µl
Buffer	2.5	1
MgCl2	2	0.8
DNTP´s	0.5	0.2
P1492R	0.5	0.2
P27F	0.5	0.2
Taq	0.25	0.1
DNA	2	0.8
H2O	17.75	6.8
Final Vol	26	10

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