June 19
Collection of soil and water samples from LA River
June 20
Lab walkthrough
Setting up lab, stock supplies, preparation of LB-amp plates
Test extraction of DNA from BioBrick wells into cloning plates
Enrichment cultures of collected samples on minimal media with BPA
June 21
MoBio kit extraction of DNA from collected samples
Enrichment cultures of samples in minimal media with 17α-estradiol, nonylphenol, and DDT
Geneious and primer design tutorial
June 22
Miniprep and sequencing of selected BioBricks (K123000, K123001, K123002, K123003)
Preparation of competent cells
June 23
PCR, digest, ligation and transformation of human estrogen receptor BioBrick to remove intron
Primer design for constructs needed to test estrogen receptor
Transfer samples from enrichment cultures to new cultures
Start cultures of RFP, transformed HER, T7 polymerase
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