Sensor: Week 5 June 12-17

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Contents

Sunday 12 June 2011

cI Repressor Test Assembly, Day 18

The Sensor group ran PCR of the K648000+K648001 using taq polymerase. The PCR products will be inserted into the OR vector.

Monday 13 June 2011

cI Repressor Test Assembly, Day 19

     The assemblies that were cultured on 6/10/11 (J23113+B0030+C0051, J23113+B0031+C0051, B0032+C0051 were sent to sequencing. The results showed that only the vector grew, meaning that the assemblies will have to be performed.

     The OR site was placed in front of the cI repressor testing construct assembled on Monday, 6/6/11 as well as in front of a terminator and mCherry reporter.

Assembly Step Part Involved with Assembly Step
Restriction Digest Insert Using SpeI and PstI: OR
Vectors Using XbaI and PstI: K648000+K648001
B0034+mCherry
Ligation OR + K648000+K648001
OR + B0034+mCherry
Transformation/Plating The above ligations were transformed into
Escherichia coli cells and plated
onto Kanamycin resistant plates.




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