Team:Arizona State/Notebook/September

 

Thursday, September 1

• Started to develop Listeria Innocua project component.

• Listeria CRISPR only uses cas9 tracr gene
• Project requires:

• cas9
• Duet plasmid
• Listeria T7 RSR
• Ligate all together in biobrick
• Test with protospacer

Saturday, September 3

• 3 PCR reactions using LI as template
• Samples visualized on gel

• sample appears slightly higher than expected

• some distortion and low resolution

Sunday, September 4

Monday, September 5

Tuesday, September 6

Wednesday, September 7

Thursday, September 8

Friday, September 9

• cas9 PCR products restricted overnight

Saturday, September 10

• 9/10 restriction products dephosphorylated for 2 hours
• Overnight ligation of dephosphorylated restriction products

Sunday, September 11

• 9/8 restriction - 9/10 ligation products transformed into NEB cells

Monday, September 12

Tuesday, September 13

Wednesday, September 14

Thursday, September 15

Friday, September 16

Saturday, September 17

Sunday, September 18

Monday, September 19

Tuesday, September 20

Wednesday, September 21

Thursday, September 22

Friday, September 23

Saturday, September 24

Sunday, September 25

Monday, September 26

Tuesday, September 27

== Wednesday, September 28 ==