Team:Johns Hopkins/Team/Attributions

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VitaYeast - Johns Hopkins University, iGEM 2011

Attributions

Media and content

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Equipment and materials

Violacein oligonucleotides were supplied by the [http://www.bs.jhmi.edu/MBG/boekelab/ Boeke lab].

A strain of yeast containing genes from X. dendrorhous on which our beta-carotene production building blocks are based was provided by the Ooyen lab. See Verwaal R, Wang J, Meijnen JP, et al. High-level production of beta-carotene in Saccharomyces cerevisiae by successive transformation with carotenogenic genes from Xanthophyllomyces dendrorhous. Applied and Environmental Microbiology. 2007;73(13):4342–4350.

We thank Dr. Tom Ellis (http://www3.imperial.ac.uk/people/t.ellis) for providing yeast plasmids and advice for quantifying the transcriptional strengths of our yeast promoter BioBricks.

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