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Team:Washington/Magnetosomes/Future
From 2011.igem.org
Future Direction
We will keep working on adding more components into the toolkits at UW and hope that future iGEM teams will be able to use toolkits to join in on the fun! As mentioned in the introduction, the purpose of this part of the project is to bring cloning from single gene level to multi-genes level to increase the complexity of the circuit that can possibly be constructed.....
- Gibson Assembly Toolkit
We have developed and submitted several vectors that are Gibson Cloning friendly(see the "parts submitted" page). More of such vectors should be developed and added to the toolkit in the future.
- Magnetosome Toolkit
We still have a long way to achieve our goal of making magnetic E.colo but this project is certainly worth investigating. We have a ton of project ideas for the future teams...
- Express the rest of the gene in the MAI region in E.coli
- Co-express the genes and study their interaction
- Build the scaffold structure in E.coli
- Express the full assembly in E.coli
- Develop assay for the magnet formation
- Determine the optimal cell growth condition......
