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Team:Kyoto/Digestion
From 2011.igem.org
Contents |
Project Digestion
Introduction
Streptomyces is a kind of prokaryotic bacteria which decompose bodies in nature. We extract protease and chitinase genes from this bacterium and introduce into Escherichia coli. Secretion-signal sequences are included in these genes so that the proteins coded by them will go out without occurring cell lysis. After assembling all genes, we examined the activity of these two enzymes in both of qualitative and quantitative ways.
Method
Construction
Assay
Serine Protease
- Experiment 1 : Skim milk-hydroryzing assay
- In order to identify the expression of SAM-P20 gene, a skim milk-hydroryzing assay was performed. A plate containing 2% skim milk, IPTG(final concentration, 0.5mM), and 0.1% yeast extract was used for this assay.
- Experiment 2 : Measurement of enzyme activity
Chitinase A
