Data page
How does E.D. Frosti work?
The first illustration is a global overview of our project in which you can see how the plasmids (and the parts) function in our system. The next illustration shows the plasmids in more detail, with the favorite new parts and the characterized pre-existing parts.
Plasmid 1 contains the bricks necessary to induce expression of the red color (via the CrtEBI system) and ice-nucleating protein (INP) in a lactose-dependent manner. For this plasmid, the INP has been cloned (
BBa_K584024) and tested for functionality by putting it behind the constitutive (
BBa_K584027) and lactose-inducible (
BBa_K584028 ) promoter.
Plasmid 2 contains
BBa_K584020 for AFP production. We were able to put AFP behind a constitutive promoter (
BBa_K584026) but due to lack of time we could not finish the AFP testing.
Plasmid 3 contains
BBa_K584004. This is a GFP-construct to test the activity of the HybB promoter. Due to lack of time we could not test this biobrick. We wanted to engineer a biobrick in which the ribokey is controlled by the HybB promoter.
BBa_K584027 was inserted in plasmid 1 to characterize INP.
Data for our favorite new parts
1. BBa_K584020 - AFP extracellular protein generator (K.U.Leuven, iGEM 2011): this biobrick generates AFP that will be expressed extracellularly. We tested this biobrick by putting it behind a constitutive promoter (which yielded BBa_K584026 ).
2. BBa_K584024 - INP generator (K.U.Leuven, iGEM 2011): this biobrick generates INP that is expressed extracellularly. We tested this biobrick by putting it behind a constitutive promoter (which yielded BBa_K584027).
Data for pre-existing parts
1. BBa_I13453 - pBAD promoter (Endy Lab, iGEM 2005): fused a GFP reporter to this promoter (which created biobrick: BBa_K584000). The results can be found at the experience page of the promoter.
2. BBa_J23119 - constitutive promoter (Berkeley, iGEM 2006): fused a GFP reporter to this promoter (which created biobrick: BBa_K584001). The results can be found at the experience page of the promoter.
We've also characterized the following parts
1. BBa_K584004 - HybB promoter + GFP generator (K.U.Leuven, iGEM 2011): we created this biobrick but due to lack of time we could not test it.
2. BBa_K584027, Constitutive promoter + INP (K.U.Leuven, iGEM 2011): we characterized this promoter by coupling it in front of a GFP. The characterization can be found on the registry page of the original biobrick: BBa_J23119 .
3. BBa_K584026, Constitutive promoter + AFP (K.U.Leuven, iGEM 2011): For this biobrick, we used the same constitutive promoter ( BBa_J23119 ), the characterization of this biobrick was the same.
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