Team:Nevada/Notebook/June/Week1

From 2011.igem.org

Notebook

E. Coli

Cyano

Enzymology

Media
Week 1 - June 1-4
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E. Coli
Blah

Cyano

Hexokinase coupled assay used to quantitate amounts of cyanobacteria glucose secretion. Rxn: glucose + ATP ==Hx==G-6-P + ADP G-6-P + NAD+ ==G-6-P DeH== G-6-P + NADH Reaction used Glucose assay kit (Genzyme) containing 2000U/L Hexokinase reagent and 4000U/L G-6-P DeH and measured glucose concentrations ranging from 0.05mM-0.25mM and absorbances were measured at 340.0 nm. Results: Final NADH concentrations were determined using the molar extinction coefficient and were proportionate to intial glucose concentrations. Discussion: Focus is now to measure not only glucose, but fructose concentrations as well as both with be secreted in the form of sucrose using a D-glucose/D-fructose assay (Megaenzyme)

Media
Week 2 - June 5-11
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E. Coli

Cyano

Enzymology

Media
Week 3- June 12-18
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E. Coli

Cyano

Enzymology