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Team:BU Wellesley Software/Notebook/VanessaNotebook
From 2011.igem.org
Week 1: 6/1-6/10
The first few days, the group sat through a boot camp, where we learned about biobricks, the basics of Biology such as DNA transcription and translation, and how to use Clotho tools. As exposure to the more computer science aspect of the project, the group learned how to write a Clotho application.
As a test run, mini preps (protocol link) were performed on s few samples of biobricks that were previously plated and transformed. Most of these samples unfortunately did not yield a high concentration of DNA, except for a few. After quantifying the samples, the samples were loaded on a gel next to a ladder to confirm the DNA that was present. However, most bands did not appear on the gel after exposed to the UV light. This concluded that there was no DNA in the samples that were being looked at. Next, several transformations were made for biobrick parts such as RBS, RFP, and constituent and inducible promoters.
The transformations produced several cultures that were both dark and light in color. Plasmid preps were made of the dark and light cultures of the UV promoter. The dark culture had an okay amount of DNA concentration. However the other samples did not have a good concentration of DNA either.
There seemed to be contamination or something else growing on the plates because some of the cultures were oddly shaped and there was a pungent smell. As a result, a sample of plasmid prep culture was stained and looked under the microscope. Most samples did not have E. coli in the sample.
