Team:LMU-Munich/Lab Notebook

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Week 1

First Primers arrived. Doing lots of PCRs.

Week 2

Still doing PCRs and start restriction digests.

Week 3

First Biobrick is ready!

Week 4

Week 5

Week 6

Week 7

Week 8

Protocols

Primer


pnikA-E,N,X-fwd
GCAGAATTCGCGGCCGCTTCTAGAGTTAAGCCTTGCGATCTGCACC

pnikA-S-rev
CCGCTACTAGTAGACGATAAAAGACGCACAAGCC

prcnA-E,N,X-fwd
GCAGAATTCGCGGCCGCTTCTAGAGacggattgtatgagacatggca

prcnA-S-rev
CCGCTACTAGTAcgcaccaagtaagatggcg

luxCDrev
CTGCAGCGGCCGCTACTAGTATTAAGACAGAGAAATTGCTTGAT

PbrR-M,R-fwd
GCAGAATTCGCGGCCGCTTCTAGAGAAGAAGGAGATATACCATGAATATCCAGATCGGCGAG

PbrR-S,A-rev
AGCCTGCAGCGGCCGCTACTAGTAttaCTAGTCGCTTGGATGGGCG

pbrRT-S,A-rev
agtcactagtattaaccggttaTTACACCTGGGTAGATGGCC

pbRMut-fwd
tcgtgcgggattctccagggactgtcggactgc

pbrMut-rev
tccgacagtccctggagaatcccgcacgattgggc

ppbrA-E,N,X-fwd
AGCCTGCAGCGGCCGCTACTAGTAGGTTGCGCGTCGCAACGGAAGC

ppbrA-S-rev
GCAGAATTCGCGGCCGCTTCTAGAGCATGCGGTGCGCTTGGCAAGC

luxCDfor
GAATTCCGCGGCCGCTTCTAGATGGAAAATGAATCAAAATA

luxBfor
ATGAAATTTGGATTGTATGAAATTTGGATTGT

luxBrev
CTGCAGCGGCCGCTACTAGTATTAGGTATATTCC

luxEfor
GAATTCCGCGGCCGcttctagaATGTGACTGGGGTGAGTGA

luxErev
CTGCAGCGGCCGCTACTAGTACTATCAAACGCTTCGGTTAA

iscSfor
AgtcgccggcAAGAAGGAGATATACCATGTACGGAGTTTATAGAGC

icsSrev
agtcactagtattaaccggtctattaatgatgagcccattcg

pabAfor
AgtcgccggcAAGAAGGAGATATACCATGAAATTGCTATTAATTGATAATTATG

pabArev
agtcactagtattaaccggtTTATTACACCACTTTCAAAAAATTATTTAAC

aurFfor
AgtcgccggcAAGAAGGAGATATACCATGCCACGACACCGCGGGC

aurFrev
agtcactagtattaaccggttaTCAACGCGGCGTGTGGGGCG

ChrBAfor
AgtcgccggcAAGAAGGAGATATACCATGAACGCTCTCCCATCCTC

ChrBArev
agtcactagtattaaccggttaTCAGTGATGCAACAACGGATAGG

melAfor
gatcTCTAGAtgGCCGGCGCGTGGCTGGTCGGCAAGCCG

melArev
gatcACCGGTGGCGGACACTATGGCTATTTCTAGC


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