Team:UT-Tokyo/Data/Method

From 2011.igem.org

Revision as of 12:16, 15 September 2011 by Ihori (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Reagents

LB broth

reagents	final conc.	amount
Bacto trypton	1%	1g
NaCl	0.5%	0.5g
Yeast extracs	0.5%	0.5g
MilliQ	-	100ml
Total		100ml

SOB broth

reagents	final conc.	amount
Bacto trypton	2%	20g
NaCl	0.05%	0.5g
Yeast extracs	0.5%	5g
KCl (250mM)	2.5mM	10ml
MilliQ	-	1000ml
Total		1L

M9

50× TAE

TB

Cultures

Extraction of DNA from cells

Amplification of DNA

Analysis of DNA

Assays

Retrieved from "http://2011.igem.org/Team:UT-Tokyo/Data/Method"