Team:Freiburg/Notebook/2 August

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Contact

This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

green light receptor

transformation with quickchanged CcaS

Investigators:Julia

Miniprep

Investigator: Jakob

Miniprep

Name: Jakob	Date: 02.08.2011
Continue from Experiment: 27.07.2011 Quickchange CcaR and trafo(28.07.2011)
Project Name: Green light receptor

Documentation:

Why are you doing this experiment? Name the parts which you extract.

Need the DNA concentration of CcaR a-d

Describe your results and mistakes and measure the DNA concentration with the Nanodrop and note the results.

Sample ID	DNA conc.	Units	260/280	260/230
CcaR qc. a	121,0	ng/µl	1,84	1,80
CcaR qc. b	121,1	ng/µl	1,87	2,05
CcaR qc. c	117,5	ng/µl	1,86	2,06
CcaR qc. d	121,4	ng/µl	1,87	2,08

How did you label your probes and where are they stored?

In Julias box, CcaR (qc.) a-d

Testdigest

Investigators: Julia

Name: Julia	Date: 02.08.2011
Continue from Experiment 02.08.2011 Miniprep
Project Name: green light receptor, testdigest of CcaR with BamHI

For one reaction you need: For Mastermix: Number of samples+2extra

4μl	H₂O	20
1μl	Buffer, NEB4	5
1μl	BSA (10x)	5
0,5 μl	Enzym 1	1	BamHI
0,5 μl	Enzym 2	-
3 μl	DNA	3

10 μl total volume

Give 3 μl of DNA in an eppi and add 7μl of the mastermix.

Incubate for about 1h at 37°C.

Add 1 μl Loading dye buffer and load the gel.

DNA Gel

Investigator: Jakob

we dont see the expected edges.

To-do: Repeat testdigest and if necessery quickchange tomorrow.

blue light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME

red light receptor

transformation with Ligationreaction(pcyA+terminator) from 1st of august and the pJT122