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From 2011.igem.org

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Notebook

Week 1

PCR primers designed according to Biobrick specifications and ordered for rsaA C-terminal and WT esp. Because of the discrepancy in GC content between Staphylococcus epidermidis and Caulobacter, we also optimized the sequence for esp for Caulobacter codons for later synthesis.

Prepared competent cells of E. coli Top10.

Prepared plates of Caulobacter, Staphylococcus aureus, and S. epidermidis using three-streak method.

Template DNA for PCR prepared from colonies using [http://www.bioventures.com/products.php?cid=10005| GeneReleaser].

Week 2