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From 2011.igem.org

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Contents 1 Photopaper 2 CO killer 2.1 2011.07.04 2.2 2011.07.05 2.3 2011.07.06 2.4 2011.07.09 3 Helmersi Codon 3.1 2011.02.13

Photopaper

CO killer

2011.07.04

Today we had our meeting in the afternoon. The weather is so hot. It was like barbeque ourselves under the sun so we decided not to have meeting in the afternoon anymore. The topic today is the oxidation of CO to CO2 – we found out that Rhodospirillum rubrum can undergo Calvin Cycle to obtain their energy from sunlight and use CO2 as their carbon source, however cyanobacteria or other bacteria is needed for CO2 fixation and production of biofuels. But it would be much more sophiscated if we add on the cyanobacteria into the system. Hmmm … looks like we have to improve more on our system design !

2011.07.05

We talked to the microbiologist Prof. Chen CY yesterday evening and stunned us with 3 questions. The time factor, the mutation factor and the gene expression rate. The experiment of CO2 fixation requires at least half a year to complete so we probably couldn’t just simply add in our project. Besides that, we also have to think of the mutation of the gene. If the gene mutated then the product would be useless. And the most important is the rate of the protein to turn fluorescent. Lastly, if the gene size is getting bigger it would be more difficult to insert into the plasmid. Consequently, we would need more time in cloning and that would be time-costing. Uhh … we really need to find the resolution soon ! Any way, Prof. Ingrid is really very busy. We have not see her for the past 2 weeks. Gotta check out with her lab assistant.

2011.07.06

Today we had meeting in the afternoon again. Thanks god the weather is windy. We downloaded all the sequences and CDS of the gene we need. On the other hand we also search the sources of R. Rubrum which we planned to use in our project. There is a few proffesor in taiwan doing the research about R.Rubrum. So Joanne will help the letter to them to request a sample of it. And the worst case we can buy it from biotech corporation. Steven need somebody to be the in-charge of wikipedia. Leo volunteered. Found out that 2 enzymes are available : CODH–Nickel, OM5 –Molybdenum; CODH is has a higher rate of binding with CO, but OM5 can bind with CO in enormous amount at one time. Say no to procrastination ! Next meeting on Friday. A day off tomorrow =D

2011.07.09

Discussion regarding the presentation slide during the preliminary round. Task allocated to all the members. While Joanne and Steven had to write the project proposal ASAP. Not much were done today. But TGIF !! Had some photo session after the meeting. Joanne go crazy with the effect thingy on the MacBook. Stressed up after one week of intensive meeting.

Helmersi Codon

2011.02.13

Let the participants to propose some ideas:

• To make the waste become ink after bacterial reaction.
• Make the bacteria to produce combustible materials.
• Make the bacteria to emit different light.
• Edible bacteria.
• To control the shape of bacterial.
• To make the bacteria a fat eater.
• Glowing mushrooms.
• Make the bacteria to stick with dust.

Poll result: Top three projects:

• Edible bacteria.
• Glowing mushrooms.
• Combustible bacteria.

Conclusion: Let all participants to choose their project and make a group then check the extent of implementation.