26 (May 26, 2011 GDS)

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Revision as of 19:24, 5 September 2011 by Sallen (Talk | contribs)

Created TENS

4.5 mL TE and
250uL 10% SDS
250uL 2N NaOH
-Spin 4 tubes (1.5mL) 30"
-Pour off sol'n & resuspend
-Add
300uL TENS: mix
150uL NaOC: mix
-Spin 2.5'
-Transfer supernatant to clean tube and add 1mL of EtOH
-Vortex and pellet DNA by centrifugation for 2.5mL
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