6 (June 6, 2011 GDS)

From 2011.igem.org

Revision as of 20:53, 6 September 2011 by Pjpatel (Talk | contribs)

Spin 1.5 mL of overnight culture for 30 secs. in micro-centrifuge. Aspirate off all but 200uL of supernatant and resuspend the pellet by vortexing. Add 300uL of TENS and mix by inversion . The solution should become viscous add 150uL of sodium acetate and vortex. A fine white precipitate should form. Centrifuge for 2.5 minutes. Transfer the supernatant to clean tube and add 2 volumes (1mL) of room temp ETOH. Mix and pellet DNA by centrifugation for 2-5 min. Wash pellet with 70% ethanol and allow pellet to dry. Resuspend the pellet in 30uL TE. Digest 5-10uL as usual.

PNICE--5/12/11
PNICE (tKAN)--5/23/11
J04430--5/23/11
J04430--5/12/11
Trans 003-W1--5/12/11
Trans 003-W1--5/23/11

P.P. G.E. S.D.

Retrieved from "http://2011.igem.org/6_(June_6,_2011_GDS)"