Team:Warsaw/Safety

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Safety

  1. Would any of your project ideas raise safety issues in terms of:
    • researcher safety,
      • Reasearchers safety:
      Work in a microbiology lab enviroment:
      In the lab there is work with GMO and E.Coli bacteria strains
      Researchers have to wear lab coats and gloves
      Work with toxic chemical compounds:
      Ethidium Bromide can cause cancer - is used only to visualize gels
      It is not added to agarose gels before running the gel.
      Antibiotics: Chloramphenicol and Ampicillin:
      While making antibiotics solutions from the powder the mask
      and safety glasses have to be worn
      Work in the RNAse free environment:
      Hazards include RNAse cleaners like RNA zap, a mask should be worn.
      The cleaners are irritating
      Work with UV light:
      UV light is a cause of cancer therefore a UV protective mask, gloves
      and lab coats have to be worn while working with the UV light
    • public safety, or environmental safety?
      • The microbiological wastes should be properly disposed - as adviced by the university
      to ensure that no GMO organisms or toxic compounts gets to the ebviroment
      The used agarose gels have to be properly disposed
    • Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues?
       No, currently our planned BioBricks are completly biosafe.
      We use only construct expressing fluorescent proteins.
      The new brick inclused modified RBS parts
      To our best knowledge those bricks are safe to use in the lab
    • Is there a local biosafety group, committee, or review board at your institution?
      In Poland there are no Biosafety groups locally at the universities. We are following Biosafety guidelines of Polish Ministry of Environment. Our department also obtained the necessary permission to work with GMO organisms.
      Therefor it is fully legal to engineer E. Coli according to Polish law
    • Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?
      • We designed a protocol to perform cloning without use of living organisms
      This protocol allows cloning biobricks without the need to produce GMO organisms.
      Therefor according to Polish law and biosafety rules it allows even high school to take part in iGEM
      The Genetically Modified Organism is create only at final stage - to test the device.
      It can be performed at GMO certified institution

 

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