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User contributions
From 2011.igem.org
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- 15:40, 18 September 2011 (diff | hist) N Team:DTU-Denmark/Transformation and selection (Created page with "{{:Team:DTU-Denmark/Templates/Standard_page_begin|Transformation and selection}} == Part 1: Transformation by electroporation == Materials: * ice * test-tubes * cuvettes * reco...")
- 15:23, 18 September 2011 (diff | hist) Team:DTU-Denmark/Protocols (→List of Protocols)
- 15:22, 18 September 2011 (diff | hist) Team:DTU-Denmark/Restriction protocol
- 15:21, 18 September 2011 (diff | hist) Team:DTU-Denmark/Restriction protocol
- 15:20, 18 September 2011 (diff | hist) Team:DTU-Denmark/Restriction protocol
- 15:19, 18 September 2011 (diff | hist) Team:DTU-Denmark/Restriction protocol
- 15:19, 18 September 2011 (diff | hist) Team:DTU-Denmark/Restriction protocol
- 15:19, 18 September 2011 (diff | hist) N Team:DTU-Denmark/Restriction protocol (Created page with "{{:Team:DTU-Denmark/Templates/Standard_page_begin|Restriction/Digestion}} == Restriction/Digestion == Materials: * 25 µL DNA (but varies depending on your DNA conc.) * 10 µL F...")
- 15:07, 18 September 2011 (diff | hist) Team:DTU-Denmark/Protocols (→List of Protocols)
- 15:06, 18 September 2011 (diff | hist) Team:DTU-Denmark/Plasmid purification by miniprep
- 15:06, 18 September 2011 (diff | hist) N Team:DTU-Denmark/Plasmid purification by miniprep (Created page with "{{:Team:DTU-Denmark/Templates/Standard_page_begin|Plasmid purification}} Plasmid purification by miniprep (Zymo Research Group) # Spin down 2 ml of cell culture in a 2 ml Eppend...")
- 15:04, 18 September 2011 (diff | hist) Team:DTU-Denmark/PCR protocol
- 15:03, 18 September 2011 (diff | hist) Team:DTU-Denmark/Ligation protocol
- 15:03, 18 September 2011 (diff | hist) Team:DTU-Denmark/Ligation protocol (→Ligation Protocol)
- 15:02, 18 September 2011 (diff | hist) Team:DTU-Denmark/PCR protocol
- 14:48, 18 September 2011 (diff | hist) Team:DTU-Denmark/PCR program design
- 14:48, 18 September 2011 (diff | hist) Team:DTU-Denmark/PCR program design
- 14:48, 18 September 2011 (diff | hist) Team:DTU-Denmark/PCR product purification
- 14:47, 18 September 2011 (diff | hist) Team:DTU-Denmark/Competent cells
- 14:46, 18 September 2011 (diff | hist) Team:DTU-Denmark/Competent cells
- 14:46, 18 September 2011 (diff | hist) Team:DTU-Denmark/Gel preparation and gel electrophoresis
- 14:45, 18 September 2011 (diff | hist) Team:DTU-Denmark/Ligation protocol
- 14:44, 18 September 2011 (diff | hist) Team:DTU-Denmark/Gel preparation and gel electrophoresis
- 14:44, 18 September 2011 (diff | hist) N Team:DTU-Denmark/PCR protocol (Created page with "== PCR protocol == PCR reaction components: a. Enzyme b. Forward primer c. Reverse primer d. dNTPs e. template DNA f. buffer g. MilliQ or distilled water Amounts per one react...")
- 14:42, 18 September 2011 (diff | hist) Team:DTU-Denmark/PCR program design
- 14:42, 18 September 2011 (diff | hist) Team:DTU-Denmark/PCR program design
- 14:41, 18 September 2011 (diff | hist) N Team:DTU-Denmark/PCR program design (Created page with "== PCR program design == Step 1: Initial denaturation for 2 minutes at 95⁰C Step 2: denature for 1 minute at 95⁰C Step 3: Anneal primers for 30 seconds at temperature ~5⁰C ...")
- 14:40, 18 September 2011 (diff | hist) N Team:DTU-Denmark/PCR product purification (Created page with "== PCR product purification == # Mix 1 volume of sample with 2 volumes of NT buffer in an 1,5 ml Eppendorf tube. # Place a column into a 2 ml collection tube and load the sample...")
- 14:40, 18 September 2011 (diff | hist) Team:DTU-Denmark/Ligation protocol
- 14:39, 18 September 2011 (diff | hist) Team:DTU-Denmark/Competent cells
- 14:37, 18 September 2011 (diff | hist) Team:DTU-Denmark/Competent cells
- 14:37, 18 September 2011 (diff | hist) Team:DTU-Denmark/Competent cells
- 14:36, 18 September 2011 (diff | hist) N Team:DTU-Denmark/Competent cells (Created page with "== Preparation of competent cells == Day 1: # Inoculate 5 ml of LB from a colony or from a -80oC stock. Day 2: # Dilute exponentially growing cells to OD600=0.05 and grow cells ...")
- 14:35, 18 September 2011 (diff | hist) Team:DTU-Denmark/Ligation protocol
- 14:27, 18 September 2011 (diff | hist) Team:DTU-Denmark/Ligation protocol
- 14:27, 18 September 2011 (diff | hist) Team:DTU-Denmark/Ligation protocol
- 14:27, 18 September 2011 (diff | hist) Team:DTU-Denmark/Ligation protocol
- 14:25, 18 September 2011 (diff | hist) N Team:DTU-Denmark/Ligation protocol (Created page with "Ligation Protocol Preparation: # Calculate your ligase concentration by estimation of DNA concentration from your restriction gel. # Which vector to use? Material: # prepare li...")
- 14:16, 18 September 2011 (diff | hist) Team:DTU-Denmark/Gel preparation and gel electrophoresis
- 14:13, 18 September 2011 (diff | hist) Team:DTU-Denmark/Gel preparation and gel electrophoresis
- 14:12, 18 September 2011 (diff | hist) Team:DTU-Denmark/Gel preparation and gel electrophoresis
- 14:12, 18 September 2011 (diff | hist) Team:DTU-Denmark/Gel preparation and gel electrophoresis
- 14:11, 18 September 2011 (diff | hist) Team:DTU-Denmark/Gel preparation and gel electrophoresis
- 14:10, 18 September 2011 (diff | hist) Team:DTU-Denmark/Gel preparation and gel electrophoresis
- 14:07, 18 September 2011 (diff | hist) N Team:DTU-Denmark/Gel preparation and gel electrophoresis (Created page with "== Gel preparation (1% gel) (100 ml of the buffer) == # 100 ml of 1x TBE buffer . # 10 μl of ethidium bomide (10 mg/ml). # 1 g of agarose. # Assembled gel container. # Mix buf...")
- 14:05, 18 September 2011 (diff | hist) Team:DTU-Denmark/Protocols
- 14:04, 18 September 2011 (diff | hist) Team:DTU-Denmark/Protocols
- 14:03, 18 September 2011 (diff | hist) N Team:DTU-Denmark/Protocols (Created page with "{{:Team:DTU-Denmark/Templates/Standard_page_begin|List of Protocols}} == List of Protocols == A number of different techniques were used for the labwork, a protocol for each of ...")
- 15:22, 6 September 2011 (diff | hist) Team:DTU-Denmark/Technical stuff lab (→Lab)
- 15:21, 6 September 2011 (diff | hist) Team:DTU-Denmark/Technical stuff lab (→Lab)
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