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Team:Freiburg/Notebook/2 September
From 2011.igem.org
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1:2 not a has inserts too! | 1:2 not a has inserts too! | ||
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| + | ===Digestion: 2A-assembly=== | ||
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| + | '''Investigators: Sophie''' | ||
| + | |||
| + | Digestion of the PCR products Notgate, Lovtap for the 2A-assembly. | ||
| + | |||
| + | ===Ligation=== | ||
| + | |||
| + | '''Investigators: Sandra''' | ||
| + | |||
| + | Ligation of the digested parts in the already digested vector pSB1C3. | ||
| + | Ligation at room temperature over night. | ||
==<span style="color:red;">red light receptor</span>== | ==<span style="color:red;">red light receptor</span>== | ||
Revision as of 09:40, 3 September 2011
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This is the wiki page of the Freiburger student team competing for iGEM 2011. Thank you for your interest!
Contents |
green light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
blue light receptor
MIniprep
Investigators: Sophie, Sandra
Miniprep of new ligated parts in pSB1C3 vector.
After miniprep samples were digested and loaded onto a gel. Not c has inserts:-)
1:2 not a has inserts too!
Digestion: 2A-assembly
Investigators: Sophie
Digestion of the PCR products Notgate, Lovtap for the 2A-assembly.
Ligation
Investigators: Sandra
Ligation of the digested parts in the already digested vector pSB1C3. Ligation at room temperature over night.
red light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
Lysis cassette
NAME OF YOUR EXPERIMENT
Investigators:NAME
Precipitator
Trafo
Investigators: Julia
Trafo of ligated parts:
- pGEX vector (Amp. resistance)
- pGEX vector (Amp. resistance)
- pGEX vector (Amp. resistance)
- pSB1C3
- pET Duet vector (Amp. resistance)
- pET Duet vector (Amp. resistance)
- pET Duet vector (Amp. resistance)
- pSB1C3
- pSB1C3
- pSB1C3
Miniprep
Investigators: Sophie
miniprep of GFP-pbd with IPTG-inducible promoter. The pellets were green!!! So we finally got both promoter and plastic binding domain into a vector! We can make experiments with the pbd now.
