Team:Bilkent UNAM Turkey
From 2011.igem.org
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investigate how nitroreductase expressing-microalgae respond to trinitrotoluene | investigate how nitroreductase expressing-microalgae respond to trinitrotoluene | ||
(TNT) exposure. Our experimental design is as follows: | (TNT) exposure. Our experimental design is as follows: | ||
- | + | firstly, obtain a synthetic gene of <i style=3D'mso-bidi-font-style:normal'>nfsI</i> with flanking prefix and suffix of standard Biobricks, and ligate this insert to pRbcBRL, a vector with | |
appropriate expression and selection system for <i style=3D'mso-bidi-font-style: | appropriate expression and selection system for <i style=3D'mso-bidi-font-style: | ||
normal'>Chlamydomonas reinhardtii</i> and obtain pRbcnfsI. Then, <i style=3D'mso-bidi-font-style:normal'>Chlamydomonas reinhardtii</i> will be transfected with the with the designed plasmid. The transfected algae will then be grown in presence of TNT and/or TNT derivatives and the effectiveness of nitroreductase activity on | normal'>Chlamydomonas reinhardtii</i> and obtain pRbcnfsI. Then, <i style=3D'mso-bidi-font-style:normal'>Chlamydomonas reinhardtii</i> will be transfected with the with the designed plasmid. The transfected algae will then be grown in presence of TNT and/or TNT derivatives and the effectiveness of nitroreductase activity on |
Revision as of 22:00, 2 September 2011