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Team:WashU/Notebook
From 2011.igem.org
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**Ura3, Leu2, KanMX4, and NatMX4 | **Ura3, Leu2, KanMX4, and NatMX4 | ||
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*Made two liters of LB solution | *Made two liters of LB solution | ||
Revision as of 17:42, 2 June 2011
This is a template page. READ THESE INSTRUCTIONS.
You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples HERE.
You MUST have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page. PLEASE keep all of your pages within your teams namespace.
| You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing. |  |
|
Tell us more about your project. Give us background. Use this is the abstract of your project. Be descriptive but concise (1-2 paragraphs) | File:WashU team.png Your team picture |
| Team Example |
| Home | Team | Official Team Profile | Project | Parts Submitted to the Registry | Modeling | Notebook | Safety | Attributions |
|---|
Contents |
Notebook
You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well.
May 31
- Preliminary Shopping List
- PCR buffer w/o MgCl - 5ML - $45.00
- M8787- 5ML -MgCl Reagent- $49.30
- D7295-.5ML - dNTP - $73.00
- D4184-250UN - Taq Polymerase - $150.00
- D8045-250UN - AccuTaq - $300
- E1385-5ML – Ethidium Bromide – $23.70
- T8280-1L – Tris Acetate EDTA Buffer – $45.10
- NA1020-1KT – PCR Clean-Up Kit -$102.50
- NA1111-1KT - Gel Extraction Kit - $102.00
Subtotal: $890.60
- Met with Cohen Lab grad students to finalize plan and get cassettes.
- Received Leu2 cassette in pRS305 plasmid.
- Received Ura3 cassette in pRS306 plasmid.
June 1
- Lesson from Bert Berla on how to design primers
- Codon Optimized the four genes using tool at: http://www.encorbio.com/protocols/Codon.htm
- Double checked gene and restriction sites to make sure enzyme does not cut in the middle of the gene
- Design Primers for cassettes
- Ura3, Leu2, KanMX4, and NatMX4
June 1
- Lesson from Bert Berla on how to design primers
- Codon Optimized the four genes using tool at: http://www.encorbio.com/protocols/Codon.htm
- Double checked gene and restriction sites to make sure enzyme does not cut in the middle of the gene
- Design Primers for cassettes
- Ura3, Leu2, KanMX4, and NatMX4
June 2
- Made two liters of LB solution
- 25g of LB Broth per Liter (10g Tryptone, 5g Yeast Extract, and 10g NaCl)
- Autoclaved solutions: 30 minute sterilization.
- Refrigerated after cooling.
