Our Approach to Biological Safety Concerns Regarding Our CRISPR Project

Identifying Safety Issues in Our CRISPR Project

Working in a lab can be fun but also dangerous if safety for the student, society, and environment are not addressed. Therefore, the safety issues of these three important entities will be examined under the scope of risk assessment. To assess risk for a biological material many factors have to be considered, which include but are limited to: pathogenicity, ability to survive outside of the lab, public awareness of the biological agent, availability of a prophylaxis, environmental impact, and ability to monitor biological material that are potentially dangerous.

pathogenicity, route of transmission, agent stability, infectious dose, concentration, origin of the potentially infectious material, availability of information, availability of an effective prophylaxis, availability of medical surveillance, experience and skill level of at-risk personnel.

Researcher Safety

Working in a laboratory is a privilege Each team member attended a Safety Orientation Course held at USC and works in a lab certified for Biosafety Level 1. All members comply with the recommended safety regulations, which include wearing gloves, goggles, and lab coats whenever necessary. Potentially harmful chemicals and devices that are used in the lab include EtBr and UV light. Both are handled with the appropriate Personal Protective Equipment.

Public Safety

Any concern for public safety is currently not anticipated. The strain of E. coli that we are experimenting with are non-pathogenic and not viable outside the lab. Materials with bacterial exposure are disinfected with bleach before they are washed. All team members wash their hands upon leaving the lab.

Environmental safety

The safety of the surrounding environment should not be compromised. The E. coli strain is non-pathogenic and are disinfected with the proper chemicals when cleaning materials.

BioBrick Safety Issues

No, our BioBrick parts and novel ones do not pose any issues in safety. All parts are being transformed in non-pathogenic E. coli and being expressed in vivo.

Biological Safety Provisions

Yes, the safety aspect of our project is being supervised by the Institutional Biosafety Committee at USC. All of the protocols are approved by the IBC at USC.

Thoughts on How to Improve Safety in iGEM

Sometimes, there seems to be a disconnection between the scientific community and the general public when discussing safety issues that stem from scientific research. With a new and rapidly evolving field that is synthetic biology entering the frame, we encourage our fellow colleagues and teams to be open-minded and communicative to the public regarding not only the benefits of the projects and synthetic biology as a whole, but also the potential harms, both physical and non-physical (e.g. morality) so that the public can be well-informed and hopefully accepting of this new science.