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Team:Tsinghua/Notebook/4 June 2011
From 2011.igem.org
(Difference between revisions)
(Created page with "pet15b digest and mini pre. File:I2011-06-04 pet15b BamHI+NdeI digestion.jpg") |
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| - | [[pet15b]] | + | ==[[pet15b]]== |
digest and mini pre. | digest and mini pre. | ||
[[File:I2011-06-04 pet15b BamHI+NdeI digestion.jpg]] | [[File:I2011-06-04 pet15b BamHI+NdeI digestion.jpg]] | ||
| + | |||
| + | gel extraction. | ||
| + | check the concentration of PW solution each time | ||
| + | blank DELL 2011-6-4 17:02:14 0.6 ng/µl | ||
| + | final dissolve DELL 2011-6-4 17:02:52 4.4 ng/µl | ||
| + | 2nd PW DELL 2011-6-4 17:03:39 2.4 ng/µl | ||
| + | 3rd PW DELL 2011-6-4 17:04:10 1.5 ng/µl | ||
| + | still get low concentration!!! | ||
Revision as of 14:36, 2 September 2011
pet15b
digest and mini pre.
gel extraction.
check the concentration of PW solution each time
blank DELL 2011-6-4 17:02:14 0.6 ng/µl
final dissolve DELL 2011-6-4 17:02:52 4.4 ng/µl 2nd PW DELL 2011-6-4 17:03:39 2.4 ng/µl 3rd PW DELL 2011-6-4 17:04:10 1.5 ng/µl
still get low concentration!!!
