Team:Osaka/week5

From 2011.igem.org

(Difference between revisions)
(→August 29(Mon))
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==August 31(Wed)==
==August 31(Wed)==
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+
# Miniprep of yesterday's culture
 +
# Restriction digests of miniprepped parts
 +
# Gel electrophoresis of digests
 +
#* 11,3-10C  ok
 +
# Ligation
 +
#* 12(C):1-1D(upstream)+1-2M(downstream)+1-3A(vector)
 +
#* 13(C):1-9N(upstream)+1-2M(downstream)+1-3A(vector)
 +
#* 14(A):3-10C(upstream)+2-18H(downstream)+1-5A(vector)
 +
#* 15(C):2-16N(upstream)+1-23L(downstream)+1-3A(vector)
 +
#* 16(A):3-10C(upstream)+3-6H(downstream)+1-5A(vector)
 +
# Transformation of ligation products
==September 1(Thu)==
==September 1(Thu)==
-
 
+
# Transfer to liquid culture
==September 2(Fri)==
==September 2(Fri)==
-
 
+
# Miniprep of yesterday's culture
 +
# Restriction digests of miniprepped parts
 +
# Gel electrophoresis of digests
 +
#* 12,13,15  ok
 +
#* 14,16  ×
 +
# Ligation
 +
#* 17(A):15(upstream)+06(downstream)+1-1G(vector)
 +
# Transfer to liquid culture:03,04,3-10C
==September 3(Sat)==
==September 3(Sat)==

Revision as of 14:13, 2 September 2011


Contents

August 28(Sun)

  1. Transfer to liquid culture:09,10,11
  2. spectrophotometry of 04


August 29(Mon)

iGEM Japan Meet-Up at Kyoto University
  1. Miniprep of yesterday's culture
  2. Restriction digests of miniprepped parts
  3. Gel electrophoresis of digests
    • 09 ok
    • 10 ok
    • 11 ×
  4. Transformaton of Registry parts (See Table 6).
Table6
IDPart NameResistanceDescription
3-10C<bbpart>BBa_K115056</bbpart>AC IPP -> OPP or DMAPP -> OPP

August 30(Tue)

  1. To liquid culture:11,3-10C

August 31(Wed)

  1. Miniprep of yesterday's culture
  2. Restriction digests of miniprepped parts
  3. Gel electrophoresis of digests
    • 11,3-10C ok
  4. Ligation
    • 12(C):1-1D(upstream)+1-2M(downstream)+1-3A(vector)
    • 13(C):1-9N(upstream)+1-2M(downstream)+1-3A(vector)
    • 14(A):3-10C(upstream)+2-18H(downstream)+1-5A(vector)
    • 15(C):2-16N(upstream)+1-23L(downstream)+1-3A(vector)
    • 16(A):3-10C(upstream)+3-6H(downstream)+1-5A(vector)
  5. Transformation of ligation products

September 1(Thu)

  1. Transfer to liquid culture

September 2(Fri)

  1. Miniprep of yesterday's culture
  2. Restriction digests of miniprepped parts
  3. Gel electrophoresis of digests
    • 12,13,15 ok
    • 14,16 ×
  4. Ligation
    • 17(A):15(upstream)+06(downstream)+1-1G(vector)
  5. Transfer to liquid culture:03,04,3-10C

September 3(Sat)

Retrieved from "http://2011.igem.org/Team:Osaka/week5"