Team:Bilkent UNAM Turkey

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Revision as of 13:20, 2 September 2011

Team Project Modelling

Deneme3 Deneme4

Lab

Notebook Lab Safety

Biobrick Parts Attributions

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Chlamy the TermiNaTor

Abstract

We aim to genetically modify unicellular microalga Chlamydomonas reinhardtii by intro ducing nfsI gene of bacterium Enterobacter cloacae in order to investigate how nitroreductase expressing-microalgae respond to trinitrotol uene (TNT) exposure. Our experimental design is as follows: obtain a synthetic g ene of nfsI with flanking prefix and suffix of standard Biobricks, and ligate this insert to pRbcBRL, a vector w ith appropriate expression and selection system for Chlamydomonas reinhardtii and obtain pRbcnfsI. Then, we can transform Chlamydomonas reinhardtii with designed plasmid. After engineering microalgae, we will grow them in t he presence of TNT and investigate effectiveness of nitroreductase activity on biological degradation of TNT.

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@@ Line 235: / Line 235: @@
 <p><span lang=3DTR>We aim to genetically modify unicellular microalga <i
-style=3D'mso-bidi-font-style:normal'>Chlamydomonas reinhardtii</i> by intro
+style=3D'mso-bidi-font-style:normal'>Chlamydomonas reinhardtii</i>  by intro
 ducing
 <i style=3D'mso-bidi-font-style:normal'>nfsI</i> gene of bacterium <i
@@ Line 249: / Line 249: @@
 tyle:
 normal'>Chlamydomonas reinhardtii</i> and obtain pRbcnfsI. Then, we can
-transform Chlamydomonas reinhardtii<i style=3D'mso-bidi-font-style:normal'>
+transform <i style=3D'mso-bidi-font-style:normal'>Chlamydomonas reinhardtii
-</i>
+ </i>
 with designed plasmid. After engineering microalgae, we will grow them in t
 he