Team:Bilkent UNAM Turkey

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<p><b style=3D'mso-bidi-font-weight:normal'><span lang=3DTR> Chlamy the TermiNaTor<o:p></o:p></span></b></p>
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<p><b style=3D'mso-bidi-font-weight:normal'><span lang=3DTR>Chlamy the Term
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iNaTor<o:p></o:p></span></b></p>
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<p><b style=3D'mso-bidi-font-weight:normal'><span lang=3DTR>Abstract<o:p></=
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presence of TNT and investigate effectiveness of nitroreductase activity on
presence of TNT and investigate effectiveness of nitroreductase activity on
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biological degradation of TNT.</span></p>
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biological degradation of TNT.<b style=3D'mso-bidi-font-weight:normal'><o:p
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Revision as of 13:17, 2 September 2011

 
Team Project Modelling
Deneme3 Deneme4
Lab
Notebook Lab Safety
Biobrick Parts Attributions

  • Chlamy the Term iNaTor

    Abstract

    We aim to genetically modify unicellular microalga Chlamydomonas reinhardtii by intro ducing nfsI gene of bacterium Enterobacter cloacae in order to investigate how nitroreductase expressing-microalgae respond to trinitrotol uene (TNT) exposure. Our experimental design is as follows: obtain a synthetic g ene of nfsI with flanking prefix and suffix of standard Biobricks, and ligate this insert to pRbcBRL, a vector w ith appropriate expression and selection system for Chlamydomonas reinhardtii and obtain pRbcnfsI. Then, we can transform Chlamydomonas reinhardtii with designed plasmid. After engineering microalgae, we will grow them in t he presence of TNT and investigate effectiveness of nitroreductase activity on biological degradation of TNT.

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