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Team:Osaka/week4
From 2011.igem.org
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# Transformation of 08 and Registry parts (see Table5) | # Transformation of 08 and Registry parts (see Table5) | ||
{|style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: #000; padding:5px;" cellpadding="5" | {|style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: #000; padding:5px;" cellpadding="5" | ||
| - | |+ | + | |+Table5 |
!ID!!Part Name!!Resistance!!Description | !ID!!Part Name!!Resistance!!Description | ||
|- | |- | ||
|4-15M||<bbpart>BBa_K325101</bbpart>||C|| EPIC Firefly Luciferase Photinus Pyralis (E. coli optimised) | |4-15M||<bbpart>BBa_K325101</bbpart>||C|| EPIC Firefly Luciferase Photinus Pyralis (E. coli optimised) | ||
|} | |} | ||
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==August 25(Thu)== | ==August 25(Thu)== | ||
Revision as of 05:54, 2 September 2011
Contents |
August 21(Sun)
- Preparation of LB agar plates (14Tet)
- Transfer to liquid culture:01,02,03,04
- Transformaton of Registry parts (See Table 4).
| ID | Part Name | Resistance | Description |
|---|---|---|---|
| 1-7A | <bbpart>BBa_J04450</bbpart> | T | construction plasmid containing mRFP coding device |
August 22(Mon)
- Colony check
- Transformed cells produced colonies!
- Non-transformed cells (negative controls) did not grow on Tet plates -> confirmed lack of natural antibiotic resistance
- Transfer to liquid culture:1-7A
August 23(Tue)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- 1-7A ok
August 24(Wed)
- Gel electrophoresis of 1-7A
- Gel extraction of 1-7A
- Ligation
- 08(T):07(upstream)+1-23L(downstream)+1-7A(vector)
- Transformation of 08 and Registry parts (see Table5)
| ID | Part Name | Resistance | Description |
|---|---|---|---|
| 4-15M | <bbpart>BBa_K325101</bbpart> | C | EPIC Firefly Luciferase Photinus Pyralis (E. coli optimised) |
August 25(Thu)
- Transfer to liquid culture:08,4-15M
August 26(Fri)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- 08 ×
- 4-15M ok
- Ligation
- 09(K):1-9N(upstream)+4-15M(downstream)+1-5A(vector)
- 10(K):1-1D(upstream)+4-15M(downstream)+1-5A(vector)
- Transfer to liquid culture:08
August 27(Sat)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- 08 ok
- Ligation
- 11(A):08(upstream)+06(downstream)+1-1G(vector)
- Transformation of 09,10,11
- Transfer to liquid culture:04
