Team:Freiburg/Notebook/29 July

From 2011.igem.org

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===Sequencing of Lysis Cassette V.2 (Quickchange-modified K098995 + Lysis genes K124017)===
===Sequencing of Lysis Cassette V.2 (Quickchange-modified K098995 + Lysis genes K124017)===
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<br>
Test Sequencing showed that the parts were assembled correctly.
Test Sequencing showed that the parts were assembled correctly.
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+
<br>
File #1: You can see the EcoRI, XbaI sites and also the Quickchange-modified K098995 as well as the XbaI-SpeI SCAR and the start of the Lysis genes K124017
File #1: You can see the EcoRI, XbaI sites and also the Quickchange-modified K098995 as well as the XbaI-SpeI SCAR and the start of the Lysis genes K124017
-
 
+
<br>
File #2: You can see part of the Lysis genes K124017 and the SpeI and PstI sites
File #2: You can see part of the Lysis genes K124017 and the SpeI and PstI sites

Revision as of 18:41, 1 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

Meeting

attendants: Julia, Manuel, Rüdiger, Sandra, Theo, Tobi Time: 9:00 - 11:00

green light receptor

already done:

  • Ccas has to sites where we need to do a mutagenesis (quick change), both were done seperately, but the no colonies showed up (wrong PCR program)
  • CcaR has one site where we need to do a mutagenesis (quick change), this has been done, colonies were growing


To-do:

  • miniprep, digestion and sequencing of the CcaR clones
  • do the Ccas PCR again
  • next steps would be the big assembly wiht pcyA

blue light receptor

already done:

  • PCR of Lov-tap and Not-gate. Not-gate sequencing was good. PCR of Lov-tap did not work the first time and the second time with the temperature gradient PCR


To-do:

  • creating new primer for the Lov-tap as the old ones had a high probabylity for secondary structures

red light receptor

already done:

  • waiting for cph8 from Mexico
  • for pcyA we did a new transformation from the original plasmid


To-do:

  • over night culture, miniprep and stock from the pcyA plates
  • 3-A-Assembly of pcyA and Terminator

Lysis cassette

already done:

  • the quick change didn't work the first time (wrong template DNA)
  • second time went well, but the transformation was difficult (only 10 colonies)

To-do:

  • do the miniprep and the sequencing

Precipitator

already done:

  • GFP-PDB: cloning doesn't work only a few colonies


To-do:

  • ask again if the gene synthesis is done: Rüdiger
  • Gibson cloning with the GST-Tag, TEV-site and Promotor-RBS


other stuff

  • deadline for the parts: 10th of september
  • name all the files you upload on the wiki with the prefix Freiburg2011_
  • start documenting the lab stuff in the wiki
  • still missing: logo and text from hiss, MPI, Serva, Invitrogen
  • Modelling: Rüdiger will ask Simon if he can help us along
  • sponsoring give-aways: key tag, postcards, movie, pencils
  • Lab tracks: Tobi wants to try it
  • cooperation with Upsala: Yeahhh!



blue light receptor

Glycerol stocks of Not-Gate

Investigators: Sandra

M45d was sequenced and we did glycerol stocks of bacteria containing M45d.



Lysis cassette

Sequencing of Lysis Cassette V.2 (Quickchange-modified K098995 + Lysis genes K124017)


Test Sequencing showed that the parts were assembled correctly.
File #1: You can see the EcoRI, XbaI sites and also the Quickchange-modified K098995 as well as the XbaI-SpeI SCAR and the start of the Lysis genes K124017
File #2: You can see part of the Lysis genes K124017 and the SpeI and PstI sites


Right-click to download the annotated ape (.gb) file #1


Right-click to download the annotated ape (.gb) file #2


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