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Team:Paris Liliane Bettencourt/Notebook/2011/07/31/
From 2011.igem.org
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pFX234/pDAG479 (200uL and 800uL) : 2 colonies <br><br> | pFX234/pDAG479 (200uL and 800uL) : 2 colonies <br><br> | ||
Cultur relaunched overday to test plasmids from Dave Lane and glycerol. | Cultur relaunched overday to test plasmids from Dave Lane and glycerol. | ||
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| + | == Camille== | ||
| + | |||
| + | Since Danyel launched a PCR yesterday, I took two of his four PCR products with Dpn1 that cut the DNA strands that is methylated. As the PCR was a quick change mutagenesis the only strand that is methylated is the one without the mutation. | ||
| + | Then the only plasmid left in the tube are the plasmid that were synthetised during the Quick change mutagenesis PCR. | ||
| + | I then desactivated Dpn1 and transformed the plasmid with the mutation in coli. | ||
| + | <br><br><br><br><br> | ||
Latest revision as of 15:52, 1 September 2011
Kevin
Results of double transformation
Witness - : no growing
Witness + (200uL) : no growing
pSG20/pDAG464 (200uL and 800uL) : 4 colonies
pFX234/pDAG479 (200uL and 800uL) : 2 colonies
Cultur relaunched overday to test plasmids from Dave Lane and glycerol.
Camille
Since Danyel launched a PCR yesterday, I took two of his four PCR products with Dpn1 that cut the DNA strands that is methylated. As the PCR was a quick change mutagenesis the only strand that is methylated is the one without the mutation.
Then the only plasmid left in the tube are the plasmid that were synthetised during the Quick change mutagenesis PCR.
I then desactivated Dpn1 and transformed the plasmid with the mutation in coli.
