Team:Freiburg/Notebook/1 September

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green light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME

blue light receptor

Picking clones

Investigators: Sandra

There were several clones on the cm plates. Clones were picked and incubated in LB cm medium at 37°C.

Gibson primer design

Investigators: Sophie, Sandra, Ruediger we designed some new gibson primers for the assembly of NOT and LOV-tap in a vector because we are not sure if we can achieve this by 3A assemblies.

red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME

Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME

Precipitator

Ligation

Procedure

PCR tube:

total volume 20 μl

1. add H₂O (17 μl -X-Y-Z)
2. add 2 μl Ligase Buffer 10x
3. add Insert 1, Insert 2(when proceeding from 3A digestion use 2 μl of each)
4. add Vector (20ng needed. When proceeding from 3A digestion use 2 μl)
5. Add 1 μl T4-DNA Ligase
6. Incubate 10-30 min at room temperature
7. heat for 20 minutes at 80°C
8. store at -20°C or directly proceed to transformation

Documentation:

Why are you doing this experiment? Where are your parts stored? Name the parts for ligation etc.