Team:Michigan/Parts

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Abstract

The ability of zinc finger domains to selectively bind specific double stranded DNA sequences have largely been applied intracellularly, such as in engineered zinc finger nucleases for genomic manipulations. Proteins containing zinc finger domains can also be used extracellularly to precisely adhere objects to surfaces containing bound oligonucleotides. This project aims to utilize the specificity of zinc finger protein to direct binding of Escherichia coli to oligonucleotides bound on surfaces. The fusion protein engineered to contain a fragment of the OmpA membrane domain and a zinc finger domain allows the protein to be expressed on the outside of the cell while remaining bound to the host cell.

Data Page

Schematic for Our System

Data For Our Favorite New Parts

1. OmpA-linker-Zif268 fusion protein controlled by LacI regulated promoter, BBa_XXXXXX (main page): This is a fusion protein assembled from OmpA-linker fusion protein, BBa_K103006 ([http://partsregistry.org/Part:BBa_K103006 main page]), and Zif268-HIV DNA binding domain, BBa_K165006 ([http://partsregistry.org/Part:BBa_K165006 main page]), controlled by the LacI regulated promoter, BBa_J04500 ([http://partsregistry.org/Part:BBa_J04500 main page]).

2. OmpA-linker-Gli1 fusion protein controlled by LacI regulated promoter, BBa_XXXXXX (main page): This is a fusion protein assembled from OmpA-linker fusion protein, BBa_K103006 ([http://partsregistry.org/Part:BBa_K103006 main page]), and Gli-1 DNA binding domain, BBa_K165007 ([http://partsregistry.org/Part:BBa_K165007 main page]), controlled by the LacI regulated promoter, BBa_J04500 ([http://partsregistry.org/Part:BBa_J04500 main page]).

3. OmpA-linker-Zif268 fusion protein controlled by AraB promoter, BBa_XXXXXX (Main page):

4. OmpA-linker-Gli1 fusion protein controlled by AraB promoter, BBa_XXXXXX (Main page):

Data For Pre-existing Parts

1. AIDA transmembrane domain, BBa_XXXXXX (Experience)

2. K113009 (araC/ pBAD)

We've Also Characterized the Following Parts

Parts

New for iGEM 2010 is the groupparts tag. This tag will generate a table with all of the parts that your team adds to your team sandbox. Note that if you want to document a part you need to document it on the [http://partsregistry.org Registry], not on your team wiki.

<groupparts>iGEM010 Michigan</groupparts>