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Copenhagen/31 August 2011
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(Created page with "==Wednesday== The plates with User2C9 had no colonies. The human CYPs are therefore to no use and are definitively dropped. '''Labwork''' * Grow cells: UserB1 and Control Bl21...")
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(Created page with "==Wednesday== The plates with User2C9 had no colonies. The human CYPs are therefore to no use and are definitively dropped. '''Labwork''' * Grow cells: UserB1 and Control Bl21...")
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Revision as of 12:20, 31 August 2011
Wednesday
The plates with User2C9 had no colonies. The human CYPs are therefore to no use and are definitively dropped.
Labwork
- Grow cells: UserB1 and Control Bl21, Took SDS sample by 0 h.
- Made Gel, ran gel with PCR products from yesterday: A2 in new expression vector (1-8) and A2 in old expression vector (1-8). Positive result for A2 new ... and A2 old ...
- Miniprep on the O/N which showed a positive result on the gel.
- Transformation of positive A2s into Bl21 cells.
- Make O/N of UserB1 to sequencing tomorrow.
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